The Preventive Effects of Oregano（Origanum vulgare) Essential Oil and Garlic（Allium sativum）Extract Mixture as Water Additives in Chickens Challenged with Eimeria tenella

• Main Authors: Wen-Hsuan Chang, 張文軒
• Other Authors: Kwong-Chung Tung
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/76617681906493160499

碩士 === 國立中興大學 === 獸醫學系暨研究所 === 102 === Coccidiosis, a protozoal disease caused by genus Eimeria in chicken, creat tremendous economic losses in poultry industry worldwild every year. Infected chicken shows weakness, poor growth performances, decreased body weight, dysentery, bloody diarrhea, or even death. Traditional methods on treatment and prevention of coccidiosis, which were based on chemical anit-coccidial agents and vaccines, have been challenged harshly by drug-resistance and drug residue issues. Therefore, many herbal extracts have been found and researched extensively such as arteannuin from Artemisia annual and polysaccharide from mushrooms. In this research, the mixture of oregano essential oil（extract from Origanum vulgare）and garlic（Allium sativum）extract were tested of the preventive effects against E. tenella on Arbor Acres broilers and Leghorn layers. Three repetitions of experiments on Arbor Acres broilers had done. 50 birds were separated into 5 groups: negative control group（NEG), positive control group with 50,000 oocysts inoculated（POST50,000), preventive group with 50,000 oocysts inoculated（PRE50,000), pos8ritive control group with 5,000 oocysts inoculated（POST5,000）and preventive group with 5,000 oocysts inoculated（PRE5,000）respectively. The experiment on Leghorn layers devided 36 birds into 3 groups: negative control group（NEG), positive control group with 50,000 oocysts inoculated（POST50,000）and preventive group with 50,000 oocysts inoculated（PRE50,000). Inoculation was performed to 2-week-old chickens. Mixture of oregano essential oil and garlic extract which diluted 10,000 times into drink water had fed continuously for 3 days before inoculation of preventive groups in both experiments. Lower O.P.G. counts had shown in preventive groups of experiments on Arbor Acres broilers. Even though without statistically significancy, the measurements of body weight gain, intestine weight ratio and cecal lesion score in preventive groups were also better than positive control groups. Result of experiment on Leghorn layers also shown lower O.P.G. counts in preventive groups. Conclusively, 0.1％ mixture of oregano essential and garlic extract in drinking water could prevent E. tenella infection in Arbor Acres broilers and Leghorn layers, especially in O.P.G. counting, which makes this product potentially become a newly natural anticoccidial combination.