Detection and application of anther culture-derived plants of Echinacea purpurea (L.) Moench

• Main Authors: Shin-Jie Huang, 黃馨潔
• Other Authors: Chung-Li Chen
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/67346612983848043243

碩士 === 國立中興大學 === 農藝學系所 === 102 === Abstract Echinacea (Echinacea spp.), commonly known as coneflower, is a North American native genus of perennials plants. One species, E. purpurea or the purple coneflower, is a very popular ornamental and medicinal plant, and has been subject to intensive breeding efforts. Commercial production of Echinacea mainly propagated by seed, however, Echinacea with the characteristics of self-incompatibility and protandrous, mainly through insects as pollinator. For cross-pollinated plants, it might form a heterogeneous group, propagated by seed will lead to agronomic traits and composition variation, hard to control quality. In the present study, anther-derived plants of E. purpurea were evaluated by chromosome number examination of root-tip, flow cytometry, morphological character discrimination and inter simple sequence repeat (ISSR) markers. Results of chromosome number and chromosomal DNA content confirmed that all of anther-derived plantlets were diploid. Morphological traits showed that they were not identical in selfing progeny. ISSR analysis of parents, anther-derived plant, self and cross-pollinated offspring demonstrate that first lot of anther-derived plantlets were not a homogeneous doubled haploid. However, some of second lot of anther-derived plantlets could be potentially homogeneous doubled haploid by ISSR analysis. To evaluate the degree of self-incompatibility by index of self-incompatibility among first lot of selfing and crossing materials, results indicated that two plantlets and four plantlets belongs to completely self-incompatible and mostly self-incompatible, respectively.