Sequentially Trapping Single Particles in Microfluidic Network

• Main Authors: Chien-Tsung Chuang, 莊建宗
• Other Authors: 陳志敏
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/05226404212903414913

碩士 === 國立中興大學 === 機械工程學系所 === 102 === In this study, the hydrodynamic filtration technique was employed for the basic design along with the theory of sequential trapping under the premise to be completely blocked. The microchannel structure was designed according to the equivalent flow resistance network system. The channel structure for separating and trapping single particles was made of Polydimethylsiloxane (PDMS) using the lithography and soft lithography techniques. Two flow inlets were employed in the experiment, one with particles and the other without particles. The inlet flow with particles was arranged to travel adjacent to the channel wall connected to branch channels for better filtration. In the experiments, single microbeads of 15 and 25 μm in diameter were successfully trapped sequentially at a total flow rate of 1.2 μL/min with the flow ratio of with and without particles at 1/5. This device has great potential applications in single cell study.