Inflammation alters vitamin B6 metabolism in vivo.
碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 102 === Abstract Rheumatoid arthritis (RA) is a systemic autoimmune disease with unclear etiology and pathogenesis. Pyridoxal 5’-phosphate (PLP) is the major B6 vitamins form, and a variety of disease conditions have repeatedly been found to be associated with low...
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| Format: | Others |
| Language: | en_US |
| Published: |
2014
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| Online Access: | http://ndltd.ncl.edu.tw/handle/p9z4pf |
| Summary: | 碩士 === 國立中興大學 === 食品暨應用生物科技學系所 === 102 === Abstract
Rheumatoid arthritis (RA) is a systemic autoimmune disease with unclear etiology and pathogenesis. Pyridoxal 5’-phosphate (PLP) is the major B6 vitamins form, and a variety of disease conditions have repeatedly been found to be associated with low levels of plasma PLP. Inverse associations have been shown between plasma PLP and biomarkers of human inflammation. Collagen-induced arthritis (CIA) and adjuvant-induced arthritis (AA) rats are commonly used as models for studying human with rheumatoid arthritis (RA). The objective of the present study is to investigate vitamin B6 metabolism during inflammation in vivo. Twenty-two 6-week-old female Lewis rats were housed in individual IVC cages under pathogen-free condition on a 12-hour day/night cycle and fed with the AIN93M diet during the experimental period. Arthritis was induced at the age of 10-week and were sacrificed at the age of 15-weeks during chronic inflammation. The volume of paw significantly increased in both AA and CIA groups during inflammation while body weight significantly decreased. Arthritis induction did not alter 24-hour urinary excretion of vitamin B6 in both models. However, inflammation resulted in drastic alterations in vitamin B6 in both liver and certain extra-hepatic tissues except for the spleen. The lower circulating plasma PLP levels observed in the CIA group reflected a decrease in hepatic PLP contents, as a positive correlation was observed between the two (r=0.731 p=0.039, n=8). The mechanism by which arthritis causes vitamin B6 mobilization during inflammation is under investigation.
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