| Summary: | 博士 === 國立中興大學 === 物理學系所 === 102 === The nano-machanical characteristics of membrane skeleton of erythrocytes on mica and PDMS substrates have been studied with Atomic Force Microscopy(AFM). It has been found that erythrocytes could be self-assembled on mica and PDMS substrates. The adhesion forces on the surface and the interactions between erythrocytes and substrates have also be measured and discussed in this study. The adhesions and motilities associated with erythrocytes and the cytoskeleton have been measured directly in liquid at nano-scale level on patterned PDMS substrates with AFM. Compared with mica, non-patterned PDMS, the patterned PDMS has been demonstrated to be more suitable for bio-sample study with AFM.
Following , we used masks to fabricate the patterns for in vivo dynamical AFM study. The interfacial properties between organisms and bio-compatiable substrates have been discussed. By using capillary attraction and cell manipulation operation, we are able to introduce erythrocytes into the semi-opening patterned PDMS substrates. A “Pumping-Diffusion sample delivery system” has then successfully established on bio-compatiable platforms. Erythrocytes have to deform into bullet-like shapes to pass through capillaries by increasing the area of the erythrocyte surface in contact with the capillary endothelium. We found that the membrane becomes rough and hard when the deformation of human erythrocytes enhances.
Finally , the characteristics of erythrocytes in Time-independence and different pH values were investigated. In this part, AFM was used to investigate the erythrocytes fine structure and the band 3 protein of membrane as measured.
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