Synthesis and Characterization of Copper(II) SNO-Schiff Base Complexes: Their Application in DNA Photocleavage and Photocytotoxicity

• Main Authors: Wan-Chun Lin, 林婉君
• Other Authors: Chu-Chieh Lin
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/psyu2w

碩士 === 國立中興大學 === 化學系所 === 102 === Photodynamic therapy (PDT) is an emergining noninvasive treatment modality for tumors. PDT involves the activation of a photosensitizer by light in the presence of an oxygen-rich environment. Recently, transition metal complexes showing anticancer activity have been extensively used in photodynamic treatment. In this thesis, a series of copper(II) complexes [LnCu(dppn)](ClO4)] (1 - 8) (n = 1 (R = 3,5-tert-Butylbenzene); 2 (R = t-butyl); 3 (R = 3-OMe); 4 (R = 4-OMe); 5 (R = 5-OMe); 6 (R = H); 7 (R = 4-NEt2); 8 (R = 5-NO2) ) with SNO-tridentate Schiff base ligands (L1 - L8) have been synthesized and characterized by elemental analysis, UV-Vis, FT-IR, EPR and LC/MS spectroscopic methods as well as X-ray crystal structural studies. Their activity and mechanism for photo-induced cleavage of supercoiled DNA and photocytotoxicity are investigated under UVA (365 nm) and blue light (430 nm) condition. Under UVA light (365 nm) and blue light (430 nm) condition. Furthermore, cellular experiments reveal that complexes (1 - 8) bear high photo-induced cytotoxicity in HeLa and SCC15 cancer cells. These results indicate that they are highly potential cadidates for photodynamic therapy.