Microelectrode arrays for biosensor application

• Main Authors: Chien-Nung Kuo, 郭建儂
• Other Authors: 蔡毓楨
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/51411378850124077349

碩士 === 國立中興大學 === 化學工程學系所 === 102 === Microelectrodes are electrode with characteristic dimensions on the micrometer scale. Faster double-layer charging, reduced ohmic loss, increased signal-to-noise ratio and high mass-transport rates have allowed them to revolutionize electroanalytical measurements. Hydrogen peroxide biosensors are fabricated by immobilization of horseradish peroxidase (HRP) onto the surface of interdigitated microband electrodes. The interdigitated microband electrode shows an amplification factor of ~9.1 with a collection efficiency of 0.97, demonstrating a high efficiency in generator-collector experiments. The resulting hydrogen peroxide biosensor exhibits a sensitivity of 0.6238 A M-1 cm-2, a detection limit of 1.22 μM, and a linear range of 5-500 μM. The performance of the proposed biosensor was evaluated with potential interferences such as ascorbic acid and uric acid.