Avian influenza hemagglutinin vaccine production in the aquatic plant Lemna minor

• Main Authors: Shun-Chih Fang, 方舜芝
• Other Authors: Liang-Jung Chien
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/7kfs59

碩士 === 明志科技大學 === 化學工程系生化工程碩士班 === 102 === Avian influenza virus has posed a worldwide threat to human health, yet there is still no effective vaccine available. This research is to develop a safety and effective recombinant subunit vaccine against pandemic influenza virus by using aquatic plant Lemna. The selected targets is the hemagglutinin i.e.HA. To achieve the highest possible levels of productivity, the target gene codon was altered by gene optimization technology. To further evaluate its potential as a commercial vaccine, in this research we developed a bioprocess for the large-scale production and purification of Lemna-made vaccine with a BioLogic LP System. It’s immunoreactivity was analyzed by Western blotting, ELISA and blocking ELISA using MAb and positive serum. In our results, hemagglutination inhibition tests indicated that the conformation of the aquatic plant-produced HA variants was correct and the proteins were functional. The immunisation of chickens and mice with the candidate vaccines elicited HA-specific antibody responses. This new research will offer an attractive system for Avian influenza oral vaccines.