Micronization of chitosan and drug encapsulation using supercritical assisted-atomization process

• Main Authors: Hou-Cyuan Chen, 陳厚全
• Other Authors: Hsien-Tsing Wu
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/72s659

碩士 === 明志科技大學 === 化學工程系碩士班 === 102 === The chitosan (CS) and encapsulated drug particulates were prepared by a supercritical assisted-atomization (SAA) process, carbon dioxide as spraying medium, and aqueous solution (50% ethanol and 1.2% acetic acid) as solvent. Low concentration of CS solution, higher saturator temperature and pressure were favorable for reducing the mean particle the mean particle sizes of CS. And the optimal flow ratio of carbon dioxide to CS solution is 1.8. The CS particle prepared by SAA process could effectively open paracellular tight junction (TJs) of Caco-2 cell monolayer and could reversibly decrease in transepithelial electrical resistance (TEER) value below 10% of the baseline. According the cytotoxicity test, Caco-2 cell monolayer had high viability upon treated by chitosan nanoparticles after 12 hours. Encapsulated drug particulates were prepared from co-precipitatuon with the above optimal condition of SAA process, the model drug is a water-soluble drug theophylline (TPH). The effect of different mass ratio of CS to TPH (Z = CS/TPH) on in vitro dissolution test of encapsulated drug particulates was investigated in this study. The experimental results of XRD and DSC showed that the drug particulates prepared by co-precipitation of SAA process formed a solid dispersion. In vitro dissolution test confirmed that the dissolution rate of drug could be manipulated by various mass ratios during the SAA process, the different dosage form and different particle size of encapsulated drug particulates. The retarded dissolution rate of TPH could be applied to the sustained delivery.