A Study of Anti-Tumor Effects on Seven Kinds of Tumor Cells and Anti-Hepatitis B Virus Activities of Nobiletin and Tangeretin

• Main Author: 許順翁
• Other Authors: Huang Ray-ling
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/25583054531156790000

碩士 === 美和科技大學 === 生技科技系健康產業碩士班 === 102 === Nobiletin and tangeretin are two pure compounds belonging to Polymethoxylated Flavones, PMF, and have anti-inflammatory, radical scavenging activity and hypocholesterol functions. The goal of this study is to explore their anti-tumor effects on 7 kinds of tumor cells (i.e., hepatoma, breast cancer, lung cancer, gastric cancer, colon cancer, brain cancer and leukemia). MTT assay data showed that nobiletin and tangeretin have no effects on gastric cancer cells (AGS), brain cancer cells (U87) or lung cancer cells (H2981); slight inhibition on breast cancer cells (MCF-7) and hepatoma cancer cells (HepG2), intermediate effect on colon cancer cells (COLO201); but showed strong inhibition on leukemia cell line (U937) (i.e., IC50 = 8.6 ± 0.6 g/mL at 24 hours and IC50 = 7.2 ± 2.0 g/mL at 48 hours for nobiletin; IC50 = 26.7 ± 0.9 g/mL at 24 hours and IC50 = 14.3 ± 1.6 g/mL at 48 hours for tangeretin) in a time and concentration-dependent manner. Moreover, by analyzing the DNA apoptosis with ELISA method, we found clearly that both nobiletin and tangeretin indeed induced apoptosis on HepG2, MCF-7, COLO201 and U937 cells. Finally, by using wild-type hepatitis B virus producing cell line (HepG2215) and lamivudine-resistant mutant virus producing cell line (M33) to evaluate the anti-HBV effects of nobiletin and tangeretin under doses with non-cytotoxicity to both cell lines, both nobiletin and tangeretin have significantly reduced the HBsAg secretion, albeit no effects to e antigen secretion.