The Study on Synthesis of Silver Nanoparticle by Chemical Reduction Method for Antibacterial Application

• Main Authors: Ming-Wei Tsai, 蔡明暐
• Other Authors: Jung-Jie Huang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/59315569825874058058

碩士 === 明道大學 === 材料科學與工程學系碩士班 === 102 === In the study, we used the chemical reduction method to synthesize silver nanoparticles by reducing silver nitrate (AgNO3) with sodium borohydride (NaBH4) and add into the capping agent to prevent the silver nanoparticles reunion. The experimental results show that different concentration of silver nitrate, and a variety of capping agent such as polyvinyl pyrrolidone (N-vinylpyrrolidone, PVP), sodium citrate and sodium poly (naphthalene formaldehyde sulfonate, Daxad 19) will affect the growth characteristics of silver nanoparticles. The surface morphology and concentration of silver nanoparticles are measured by field-emission scanning electron microscopy (FE-SEM), transmission electron microscopy (TEM), UV-vis spectrophotometer, particle size analyzer and inductively coupled plasma optical emission spectrometry (ICP-OES) have been employed to characterize the silver nanoparticles. This study successfully prepared silver nanoparticles with a diameter of 10-90 nm and that has optimum dispersion by using Daxad 19. We control the particle size of silver nanoparticles to 7 nm, for the Escherichia coli the ability of antibacterial has more than 94 %. In the optimum condition, the antibacterial ability for Escherichia coli is more than 90% when the concentration of silver nanoparticles at 15 ppm. As the result, Ag nanoparticles have greater antibacterial effectiveness.