| Summary: | 碩士 === 銘傳大學 === 生物科技學系碩士班 === 102 === Previous study, the protein phosphatase was widespread and exist in eukaryotes. It had the ability to assist the transfer of a signal of the adversities[1]. But the role of protein phosphates in the rice still unknown. To analysis if the OsPP1 could improve the environmental adversity to tolerance, we used the Agrobacterium-mediated transformation of rice phosphatase 1 (OsPP1a) in Tainung 67. Results showed that overexpression of OsPP1a in rice can improve the expression of SnfRK1A, thereby enhancing the expression of anti-adversities gene OsNAC5 and OsNAC6 thus increased high salt and drought stress tolerance. Expression of OsPP1a also improved the expression and activity of α-Amylase and ascorbate peroxidase. These results indicate that OsPP1a involved in regulation of adversities gene, cell metabolism-related genes and antioxidant genes in rice. Conclusionly, OsPP1a might play a key role in regulation of stresses in rice.
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