| Summary: | 碩士 === 崑山科技大學 === 機械工程研究所 === 102 === Abstract
The conventional methods used in specific cell trapping are for example the use of antibody-based chip and the structure-based chips. These chips capture targeted cells by filtering the cells based on their specific surface proteins and difference in size, respectively. Major drawbacks with antibody-based chip consist of the time and cost in antibody processes. Major drawbacks with filter-based device such as Patch Clamping are the clogging problem because of the isolation purity and the viability of the captured cells. Hence, new electro kinetic technologies are rapidly being introduced to the research community as potential tools to offer spatial control and manipulation of cells in microfluidic systems. This thesis focuses on the antibody-free specific capture of cancer cell using electro kinetics-based microfluidic chip.
We present an innovative design of micro-sized particle trap that uses dielectrophoresis (DEP) to selectively trap cells in continuous flow. Different designs, with the aim to achieve the best possible result, were simulated on COMSOL. After careful study, design and changes being applied, the best suitable design was determined to be used in trapping single cancer cell from the mixture. MEMS technology was used to create the 3D structures on the silicon wafer, through deposition, etching and photolithography. Lung cancer cells were successfully trapped in the designed specific regions, thus demonstrating the excellent capability of the proposed chip. The microfluidic device presented was capable of selectively capturing single cells due to the structure design which incorporates microfluidic and DEP principles to trap single cell.
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