Using two in vitro exposure models to assess toxic effects of two chemicals in cooking oil fumes on human lung cells.

• Main Authors: Wei-Jie Huang, 黃薇潔
• Other Authors: Chiung-Yu Peng
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/3nt2qc

碩士 === 高雄醫學大學 === 職業安全衛生研究所 === 102 === Recently, a lot of evidences indicate that household and occupational exposure to cooking oil fumes (COFs) has been associated with lung cancers and other diseases. Additionally, IARC classified emissions from high-temperature frying as group 2A. Therefore, it is necessary to assess toxic effects of COFs at the cellular level to understand and clarify possible pathological mechanisms. The aim of this study was to study the biological effects of human lung cells exposed to two chemicals in COFs (formaldehyde and trans, trans-2,4-decadienal ) by using two in-vitro exposure modules. The investigated biological effects were cell viability (MTT assay) and oxidative stress (ROS analysis). For A549 cells exposed to both chemicals in the submerged culture exposure system, cell viability decreased with longer exposure time and higher exposure concentrations. Exposed to 400 μM tt-DDE or 30 mM formaldehyde would cause cell viability decreasing below 50%. Additionally, oxidative stress increased with longer exposure time and higher exposure concentrations. Our results revealed that the cytotoxicity of tt-DDE was more harmful than that of formaldehyde at the submerged culture exposure system. For A549 cells exposed to formaldehyde in the air-liquid interface (ALI) exposure system, more severe biological effects would also found under longer exposure time and higher concentrations. ALI exposure model will be an appropriate and useful exposure module in terms of representativeness of real situation for air pollutant exposure. Therefore, further researches should be carried out by using the ALI system, to provide basic information regarding the dose and time-dependent toxicity and study its effect on human lung cell.