| Summary: | 博士 === 義守大學 === 電機工程學系 === 102 === Osteoarthritis (OA) is one of the most commonly and widely occurring degenerative disorders of joints in various parts of our body. However, according the existing non-invasive examination protocols for OA, only the very severe disorders could be readily diagnostically confirmed. In other words, by examining X-ray radiograms or MRI findings for evaluating the gaps of articular cartilage in the joints, only in severe cases of OA, where the physician might be able to find obvious differences in the voids or contacts within the gaps between the healthy and symptomatic joints. In fact, the x-ray or MRI findings could not effectively provide information for the early detection of articular cartilage degeneration. In the present study, we have developed a diagnostic test strip, based on the concept of Amperometric Immunoassay methodology, to determine the concentration of patient’s urinal fluid which containing COMP biomarker for OA.
We divided the study into two parts: first part for development of the urine strips, and second part for the application of the urine strips in clinics.
The first part of the study is the preliminary results demonstrated that the optimal conditions to prepare the test strip were: 60-70 μg/ml of antibody to combine the nano-gold, the optimal concentration of antibody was 1.5 μg and the optimal reaction time was 15 minutes. These preliminary prepared test strips were with 92.8 % accuracy from 70 patients’ tests (From E-DA hospital). Also, we have established a clinical degenerative index for the OA knee patient from E-DA hospital and used this index as an OA reference for this present and further study.
The second part is to use the developed urine strips for the different grade of knee OA to evaluate the clinical correlation with the result of urine strips. Although, the urine samples from total 71 patients with knee OA were collected from E-DA hospital. 50 samples (70.4%) have positive results after examination by test strips. We still need more number of patients to evaluate and identify the clinical application of the developed urine strips. To date, we have met the commitment of preparation and test of test strip for OA, however, the curvet preparation and test need more periods to complete.
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