Study of Nano-sized Collage I Molecules combined with Nicotinamide and Exendin-4 on Differentiation of MSCs into Insulin-Secreting cells

• Main Authors: Yi-Jhen Wu, 吳宜真
• Other Authors: Shyh Ming Kuo
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/yvw3s4

碩士 === 義守大學 === 生物醫學工程學系 === 102 === Recent research reports indicated that MSCs could be differentiated into Insulin-secreting cells by Nicotinamide. However, these studies focused on the usage of pure Nicotinamide chemicals and lacked details regarding the influence/contribution of ECM in combination with induction chemicals. Also many related researches focused on the preparing bio-mimetic scaffolds very limited imevaluated the influence of nano-sized order ECM on the cellular interactions. The motivation and purpose of this study are to develop a different configuration of the collagen, and combine the induction chemicals, Nicotinamide and Exendin-4 with the differentiation of MSCs into insulin secreting cells. The adhesion and interactions between the cellular receptor and nano-sized collagen molecules, and the probably contribution of these molecules on the differentiations of MSCs into insulin secreting cells will also be included. Established animal experimental model of islet defect, then between mesenchymal stem cells cultured in vitro induction of insulin secretion cells implanted into the pancreas conducted to assess the regulation of insulin secretion function. Preparation collagen form of fibers and nanoparticls biological molecules, explore receptor extracellular matrix molecules adsorbed on the cell membrane, and combined Nicotinamide and Exendin-4 will be the application of mesenchymal stem cells into insulin secreting cells cultured. From the cells results, can be observed after culture mesenchymal stem cell morphology gradually induced into bunches type of islet cells. After adding the type I collagen and nano-fibers, mesenchymal stem cells into islet cells than simply the Nicotinamide and Exendin-4 multi-inducing and obvious. Display type I collagen molecule is effective promote differentiation between mesenchymal stem cells. Results are shown in immunofluorescence, mesenchymal stem cells were cultured for 14 days before observation of the red protein expression of insulin, significantly increased expression of insulin. Analysis of the results type I collagen molecules are nanoscale between promoting effects of mesenchymal stem cells to differentiate into islet cells. On the results of animal experiments, rats fed high-fat diet, and after intraperitoneal injection of STZ, after a glucose tolerance test, glucose - insulin tolerance test and glycosylated hemoglobin index after determining a second type can be induced diabetic rats and completion of the insulin-secreting cells induced transplanted pancreas, and hematoxylin - eosin staining. And the establishment of animal experimental model of islet defect, then between mesenchymal stem cells cultured in vitro induction of insulin-secreting cells were implanted into the pancreas, to assess the function of regulating insulin secretion.