| Summary: | 碩士 === 義守大學 === 化學工程學系暨生物技術與化學工程研究所 === 102 === Response surface methodology (RSM) was applied to optimize the extraction conditions for anti-osteogenesis activity of Glossogyne tenuifolia. Five operation factors were investigated, including extraction time, agitation rate, alcohol concentration, temperature and solid-solution ratio (SSR). The extraction yield, the anti-osteogenesis activity and the desirability that combines these two indexes were the three objective functions. The anti-osteogenesis activity of G. tenuifolia extract was assayed by tartrate resistant acid phosphatase (TRAP) activity and expressed by the inhibition concentration (IC). At first, the experiments of fractional factorial design (FFD) and the path of steepest ascent were performed to search for the optimum operation area for TRAP activity. Then, the central composite designed (CCD) experiments were carried out to search the optimal extraction conditions. Three second-order equations were obtained by regression via each of the three objective functions, and the equations were used to predict the optimum points. The factor analysis from these equations showed that the most important factor on each of these three objective indexes was all the alcohol concentration. When the extraction yield was the objective function, the highest yield (20.3%) was obtained, but the anti-osteogenesis activity was low (IC = 77.0 g/ml). When the anti-osteogenesis activity was the objective function, the highest anti-osteogenesis activity (IC = 46.0 g/ml) was obtained, but the extraction yield was low (12.1%). If the desirability function was used as the optimization index, a compromise result (yield = 19.9%, IC = 63.1 g/ml), that may take into account the production cost and health activity, was obtained. To compare this extract with a non-optimized extract, the TRAP activity decreased from 80% to 47.1% at a dose of 25 μg/ml, and the extraction yield increased from 14.7% to 19.9%.
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