Using Image Analysis Technology to Evaluate the Dynamic Behavior of Stem Cells in Different Culture Environments

• Main Authors: Ka-Pei Tsai, 蔡克培
• Other Authors: Ching-Fen Jiang
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/98750507194255870305

碩士 === 義守大學 === 生物醫學工程學系 === 101 === In recent years, the focus of regenerative medicine research has been on vitro cultured stem cells. During the cultivation processes, differences between the culture environment and the cultivation methods can easily influences cell viability. Therefore, quantitative analysis is needed to investigate the relationship between cell viability and culture conditions. Therefore, this study developed four modes to analyze the dynamic behavior according to different cell features. The modes aim to deal with images with good contrast, poor contrast, incomplete contrast, and fluorescent staining. First, while selecting the appropriate mode to analyze cell images, the program will use morphological operations to solve the uneven illumination problem, or perform contour reconstruction using cell features. Next, cell tracking in accomplished by searching for the best correspondence of the cell centroids between the successive frames. Finally, cell activity parameters are calculated for the evaluation of the dynamic behavior of the cells. The results of this study show significant variation in cell area and contour irregularity in the aggregation process. Moreover, validation results show 85% of consistency in cell segmentation in comparison with manual drawing and 100% of tracking accuracy. Therefore, the system can assist the biomaterials developer to quantitatively assess cell viability for cell culture environment adjustment.