The Senescence Prevention Mechanism Studies of Dietary Selenium Supplement on Kidney of Aged Rats

• Main Authors: Ya-Ping Hau, 許雅萍
• Other Authors: Shyh-Mirn Lin
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/68931555592447131305

碩士 === 中華醫事科技大學 === 醫學檢驗生物技術系碩士班 === 102 === Kidney aging and its function degradation, compared to other organs, is more obviously. Its degradation also affects the function of other organs. Selenium (selenium, Se) necessary for the human body and exhibit the effects of aging prevention were reported. In this study, two different types of selenium (inorganic selenium and organic selenium) were used to explore the potential prevent aging mechanisms in aging rats mode. Total 39 of 79-week-old male Sprague-Dawley rats were divided into five groups, and fed different experimental diets separately : (1) the aging control group (older control, OC), (2) low-dose inorganic selenium group (low selenite, LSE; 0.5 mg / kg), (3) high-dose inorganic selenium group (high selenite, HSE; 2 mg / kg), (4) low dose of organic selenium group (low selenium yeast, LSY; 0.5 mg / kg), (5) high-dose organic selenium group (high selenium yeast, HSY; 2 mg / kg), and compare with a young control group (young control; YC). All groups were sacrificed and analyzed after 14 weeks feeding. Biochemical markers of kidney disease in the blood such as Blood urea nitrogen (BUN) was found that 33.24 mg / dL of OC group was significantly higher than 22.98 mg / dL of YC group, and each group with selenium supplementation were decreased to 30 mg / dL or less. In Creatinine Clearance (CCr), 0.65 mL / min of OC group was less than 1.09 mL / min of YC group, and each group with selenium supplementation could increase to 1 mL / min. From the biomarker assays, selenium supplements could improve the physiological function of the kidney. In the systemic lipid peroxidation index evaluation, the 8-isoPGF2α level of 32.79 ng / day in OC Group was significantly higher than 15.84 ng / day in YC group. However, each group with selenium supplement could significantly reduce lipid peroxidation approximately 20 ng / day. The antioxidant enzyme activity of catalase, GPx, SOD, GSH in the groups of selenium supplement were also found higher than in OC groups. These results reflect selenium could enter blood circulation and exert antioxidant activity. In addition, proteomic study by two-dimensional electrophoretic was used to explore the differences between the control group and selenium supplement group. Results showed there were significant difference in endoplasmic reticulum stress-related proteins, such as Transitional Endoplasmic Reticulum ATPase and Heat Shock 70kDa protein 5. The level of endoplasmic reticulum stress-related proteins expression in OC groups was less than in YC group, and the level of protein expression in the groups with selenium supplementation were found close to YC group. The results show that the stress of endoplasmic reticulum should associate with aging. Furthermore, the correlation of selenium added and endoplasmic reticulum stress-related proteins expression was conducted in a salubrinal-induced stress in NRK-52E renal epithelial cells model. The level of endoplasmic reticulum stress-related proteins expression, such as PERK, calnexin, GRP78, PDI and ERO1, were found to be enhanced after the selenium addition. This demonstrated that selenium supplements should be able to maintain the structure integrity of ER-stress proteins. Based on these regards, selenium supplements should have the potential used in kidney aging prevention.