Research on Preparation of Lotus (Nelumbo Nucifera Gaertn.) Seed Protein Hydrolysates and Their Antioxidant Activities

• Main Authors: Chang Chien Ru, 張茜茹
• Other Authors: 呂君萍
• Format: Others
• Language: zh-TW
• Published: 2015
• Online Access: http://ndltd.ncl.edu.tw/handle/62079939380669140845

碩士 === 輔仁大學 === 食品科學系碩士班 === 103 === Abstract Lotus (Nelumbo nucifera Gaertn.) seeds are widely used as traditional medicine in Asia. The average moisture, ash, protein, fat, and carbohydrate contents were 4.3%, 5.5%, 21.9%, 2.4%, and 65.9%, respectively, on a dry weight of powder. The lotus seed protein isolate (LSPI) was then enriched with 93.9% of protein content and a 10.2% final yield was obtained. The LSPI was hydrolyzed using commercial protease (alcalase and flavourzyme) to evaluate antioxidant activities by DPPH scavenging ability, reducing power, ferrous chelating, and hydrogen peroxide scavenging. At all antioxidative assays, antioxidative activities increased with increasing DH, and exhibited significantly difference compared to LSPI. The highest degree of hydrolysis was found after 180 min of hydrolysis with alcalase and flavourzyme. There was a dose-dependent relationship between hydrolysate concentration and antioxidant activity. The hydrolysate (DH 180) prepared from flavourzyme possessed the highest scavenging activities on DPPH (EC50 2.96 mg/mL), ferrous chelating (EC50 4.34 mg/mL), reducing powder, and hydrogen peroxide scavenging activity (EC50 > 16.10 mg/mL). The SDS-PAGE data showed a range of low molecular weight peptides generated after hydrolysis and may contribute to antioxidant capacities. Therefore, lotus seed protein hydrolysates could be used as a potential bioactive ingredient for preventing production of reactive oxygen species. Keywords: Lotus seed, protein hydrolysate, protein isolate, antioxidant