PRP-derived Growth Factors Released From Oxidized Chondroitin Sulfate-Gelatin Promote Chondrogenic Differentiation Of Synovial Fluid Mesenchymal Stem Cells

• Main Authors: Tay Yeung Ping, 鄭詠彬
• Other Authors: Hsia-Wei Liu
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/96051228366230212626

碩士 === 輔仁大學 === 生命科學系碩士班 === 102 === Title：PRP-derived Growth Factors Released from Gelatin-oxidized Chondroitin Sulfate Hydrogels Promote Chondrogenic Differentiation of Synovial Fluid Mesenchymal Stem Cells. Keywords：PRP; Hydrogels; Synovial mesenchymal stem cells; Chondrogenic. Background：Currently research in tissue engineering, hydrogel can be used as a drug delivery system, cell carrier and scaffolds. Several polysaccharides are partially oxidized and employed for possible medical applications such as drug release or cell carrier. Aim：In this study we report a new class of biodegradable composite hydrogels which derived from oxidized chondroitin sulfate and gelatin upon mixing, without the addition of a chemical crosslinking agent. Methods：The gelation of oxidized chondroitin sulfate and gelatin was linked between amino and aldehyde groups of polysaccharide derivatives through the Schiff base reaction. MTT assay used to cell viability analysis. And total collagen content, glycosaminoglycan content was examined. on cell differentiation. Results：Oxidized chondroitin sulfate and gelatin as a biodegradable hydrogel, the oxidation of chondroitin sulfate was 55%、82% and 92%. The ratio of Oxidized chondroitin sulfate and gelatin was 2:8, 3:7, 4:6 that is 8G2CSD55、7G3CSD55、6G4CSD55、8G2CSD82、7G3CSD82、6G4CSD82、8G2CSD92、7G3CSD92、6G4CSD92。The relation of pH and gelation time was the gelation time of pH 11 shorter than pH 7, the higher ratio of gelatin affect the gelation time become longer. The size of poles of hydrogel was 30μm to150μm under SEM. Water content of hydrogel between 9 to 11%. The growth of cell have a higher viability on hydrogel. Total protein was increase in day7 to day14 at cell MC3T3. The higher oxidation chondroitin sulfate the of lower of total collagen. Conclusion：The experimental results show that the gelation of 92% oxidation rate oxidized chondroitin sulfate and 4% gelatin through the Schiff base reaction at pH11 as a control released carrier of synovial fluid mesenchymal stem cell..