Characterization of Incubation and Washing Processes on Disk-based ELISA using Magnetic Beads

• Main Authors: Chang,Chung-Yi, 張仲逸
• Other Authors: Shih,Chih-Hsin
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/07861201019910716912

碩士 === 逢甲大學 === 化學工程學系 === 102 === This work characterized the incubation and washing processes on an automated, disk-based, enzyme-linked immunosorbent assay (ELISA) system using magnetic beads. The magnetic module integrated on the system is capable of conducting both incubation and washing steps automatically through disk rotation. The movement of the magnetic beads during incubation was investigated. It was found that the optimal rotational speed is independent of the size and concentration of the magnetic beads but related to the strength of the magnetic fields and the number of the magnet arrays. In addition, the factors which influenced washing process were also investigated. It was found that the washing efficiency is governed by both the entrance and exit flowrates of the reaction chamber. The best liquid replacement efficiency occurred at a situation with a low entrance flowrate and high exit flowrate.