| Summary: | 碩士 === 逢甲大學 === 化學工程學系 === 102 === In this study, the protocol of the disk-based enzyme-linked immunosorbent assay (ELISA) using magnetic beads was investigated. Conventionally, ELISA was mostly conducted using microtiter plate. Since the protocol involved many reagent adding and removing steps, it is labor-intensive and time consuming. The disk-based platform emerged as a useful alternative tool for ELISA. It has been demonstrated that by integrating the burst valve and the programmable spinning control, ELISA can be conducted in an automated way. With the assist of the magnetic beads, the assay time was reduced and sensitivity of the assay was improved. Since the space on the disk is limited, it is very important to optimize this assay protocol. The process parameters, such as wash buffer and substrate volume, concentration of the magnetic beads, and the geometry of the fluidic design were investigated. The optimal conditions were found and discussed.
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