Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production
碩士 === 逢甲大學 === 化學工程學系 === 102 === Protein synthesis in living cells is an energy-dependent process. Accordingly, the level of protein production is closely related to the energy status of cells. In this study, we enhanced glucose and glycerol metabolism in Escherichia coli. The experimental results...
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ndltd-TW-102FCU050630022019-05-15T21:13:38Z http://ndltd.ncl.edu.tw/handle/az34rn Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production 葡萄糖與甘油代謝對於重組蛋白質生產的影響 Yan-jing Chen 陳妍菁 碩士 逢甲大學 化學工程學系 102 Protein synthesis in living cells is an energy-dependent process. Accordingly, the level of protein production is closely related to the energy status of cells. In this study, we enhanced glucose and glycerol metabolism in Escherichia coli. The experimental results showed that the engineered strain performed better than its non-modified counterpart in terms of growth and the production yield of recombinant proteins. It clearly indicates that this proposed approach is promising to improve the physiological trait of producer strains for recombinant protein production. Yun-peng Chao 趙雲鵬 2014 學位論文 ; thesis 128 zh-TW |
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碩士 === 逢甲大學 === 化學工程學系 === 102 === Protein synthesis in living cells is an energy-dependent process.
Accordingly, the level of protein production is closely related to the energy status of cells. In this study, we enhanced glucose and glycerol metabolism in Escherichia coli. The experimental results showed that the engineered strain performed better than its non-modified counterpart in terms of growth and the production yield of recombinant proteins. It clearly indicates that this proposed approach is promising to improve the physiological trait of producer strains for recombinant protein production.
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Yun-peng Chao |
author_facet |
Yun-peng Chao Yan-jing Chen 陳妍菁 |
author |
Yan-jing Chen 陳妍菁 |
spellingShingle |
Yan-jing Chen 陳妍菁 Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production |
author_sort |
Yan-jing Chen |
title |
Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production |
title_short |
Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production |
title_full |
Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production |
title_fullStr |
Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production |
title_full_unstemmed |
Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production |
title_sort |
effect of glucose and glycerol metabolism on recombinant protein production |
publishDate |
2014 |
url |
http://ndltd.ncl.edu.tw/handle/az34rn |
work_keys_str_mv |
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