Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production

碩士 === 逢甲大學 === 化學工程學系 === 102 === Protein synthesis in living cells is an energy-dependent process. Accordingly, the level of protein production is closely related to the energy status of cells. In this study, we enhanced glucose and glycerol metabolism in Escherichia coli. The experimental results...

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Main Authors: Yan-jing Chen, 陳妍菁
Other Authors: Yun-peng Chao
Format: Others
Language:zh-TW
Published: 2014
Online Access:http://ndltd.ncl.edu.tw/handle/az34rn
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spelling ndltd-TW-102FCU050630022019-05-15T21:13:38Z http://ndltd.ncl.edu.tw/handle/az34rn Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production 葡萄糖與甘油代謝對於重組蛋白質生產的影響 Yan-jing Chen 陳妍菁 碩士 逢甲大學 化學工程學系 102 Protein synthesis in living cells is an energy-dependent process. Accordingly, the level of protein production is closely related to the energy status of cells. In this study, we enhanced glucose and glycerol metabolism in Escherichia coli. The experimental results showed that the engineered strain performed better than its non-modified counterpart in terms of growth and the production yield of recombinant proteins. It clearly indicates that this proposed approach is promising to improve the physiological trait of producer strains for recombinant protein production. Yun-peng Chao 趙雲鵬 2014 學位論文 ; thesis 128 zh-TW
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language zh-TW
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description 碩士 === 逢甲大學 === 化學工程學系 === 102 === Protein synthesis in living cells is an energy-dependent process. Accordingly, the level of protein production is closely related to the energy status of cells. In this study, we enhanced glucose and glycerol metabolism in Escherichia coli. The experimental results showed that the engineered strain performed better than its non-modified counterpart in terms of growth and the production yield of recombinant proteins. It clearly indicates that this proposed approach is promising to improve the physiological trait of producer strains for recombinant protein production.
author2 Yun-peng Chao
author_facet Yun-peng Chao
Yan-jing Chen
陳妍菁
author Yan-jing Chen
陳妍菁
spellingShingle Yan-jing Chen
陳妍菁
Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production
author_sort Yan-jing Chen
title Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production
title_short Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production
title_full Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production
title_fullStr Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production
title_full_unstemmed Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production
title_sort effect of glucose and glycerol metabolism on recombinant protein production
publishDate 2014
url http://ndltd.ncl.edu.tw/handle/az34rn
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