Effect of Glucose and Glycerol Metabolism on Recombinant Protein Production
碩士 === 逢甲大學 === 化學工程學系 === 102 === Protein synthesis in living cells is an energy-dependent process. Accordingly, the level of protein production is closely related to the energy status of cells. In this study, we enhanced glucose and glycerol metabolism in Escherichia coli. The experimental results...
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| Format: | Others |
| Language: | zh-TW |
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2014
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| Online Access: | http://ndltd.ncl.edu.tw/handle/az34rn |
| Summary: | 碩士 === 逢甲大學 === 化學工程學系 === 102 === Protein synthesis in living cells is an energy-dependent process.
Accordingly, the level of protein production is closely related to the energy status of cells. In this study, we enhanced glucose and glycerol metabolism in Escherichia coli. The experimental results showed that the engineered strain performed better than its non-modified counterpart in terms of growth and the production yield of recombinant proteins. It clearly indicates that this proposed approach is promising to improve the physiological trait of producer strains for recombinant protein production.
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