| Summary: | 碩士 === 大葉大學 === 生物產業科技學系 === 102 === Acne is a common skin diseases about hair follicles and sebaceous glands. There are many reasons for its pathogenesis, including excessive secretion of sebum, comedo, acne bacteria breeding and inflammatory responses etc. Acne bacteria is not only playing an important role in inflammatory responses, also the main reason of comedo becoming. But the treatment usually use antibiotic or chemical anti-inflammatory drugs those often have problems of drug resistance and side effects. Therefore, this research primarily uses the medicinal plants of Plectranthus amboinicus (Lour.) and medicinal fungi Antrodia cinnamomea applying to effectiveness evaluation of the acne treatment, and further explore anti-inflammatory effects of the two substances and combining with bacterial cellulose film that prepare into application of antimicrobial dressing materials. The first part of results are displayed P. amboinicus extract in different extraction methods without inhibition effects for acne bacteria P. acnes and S. epidermidis. In nine kinds of grains species of extraction liquid after culturing A. cinnamomea fermentation show only Ac-O extract that has a significant inhibitory effect for P. acnes and S. epidermidis. Exploring O in extraction liquid after culturing different A. cinnamomea fermentation has the best of bacteriostatic action with Ac6、Ac8 and Ac9. The second part is LPS stimulation of RAW 264.7 cells inflammatory reaction and results show the P. amboinicus extraction can effectively inhibit NO production and iNOS,COX-2 and NF-κB protein expression function with Ac extraction, they will accompany inhibition of LPS-induced inflammatory cytokine TNF-α, IL-6 production. However, the wine extract-stem in P. amboinicus and stock solution-leaf extracts under 6000 μg/mL and Ac8 and Ac9 extracts at 750 μg/mL and 1000 μg/mL concentration have a significant difference in inhibition of IL-10. The third part will preliminary study the impact of combining P. amboinicus and Ac extraction with bacterial cellulose film for the acne bacteria of P. acnes as well as S. epidermidis. The results that lyophilized bacterial cellulose film as substrate of the antibacterial effect are superior to dried bacterial cellulose film. Consolidated results can be found the P. amboinicus extraction have anti-inflammatory effect and Ac extraction have antibacterial and anti-inflammatory effects those will be potential for the development of alternative antibiotics or chemical treatment of acne medication and combining the antibacterial fiber membrane of bacterial cellulose films prepared as the application of antimicrobial dressings have good potential for development in the future.
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