| Summary: | 碩士 === 朝陽科技大學 === 應用化學系生化科技碩博士班 === 102 === Protein is an important component in feed for healthy animal growth. Currently there are shortages in most commonly used protein sources for animal protein, namely, blood meal, fish meal, bone meal etc. Vegetable protein has been considered to compensate shuch shortages. Cottonseed and its meal are good choices for such purpose. However, there have been concerns about the toxic effect of gossypol naturally occurred in cottonseed that might be harmful to the animal. Regulatory limit allowances of gossypol have been announced (e.g. 60 ppm for swine, 100 ppm for broilers). A practical solution would be the addition of gossypol binder that will adsorb gossypol and leave the animal unharmed. Due to the similarities in chemical structures between gossypol and mycotoxin molecules, mycotoxin adsorbent is a candidate in such application. This research studied the adsorption of gossypol on two types of mycotoxin binders: Calibrin-A and Calibrin-Z. Experiments were carried out in phosphate buffers. HPLC was used to quantify gossypol concentrations. Results showed that these two mycotoxin adsorbents both bound gossypol at high dosage (e.g. 90 mg / 20 mL, and 2 ppm gossypol).
|
|---|
