Investigation of Gold Nanoclusters on Early Mouse Embryonic Development

• Main Authors: Jiun-Yi Chiou, 邱君羿
• Other Authors: Wen-Hsiun Chan
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/87629592582463315816

碩士 === 中原大學 === 奈米科技碩士學位學程 === 102 === Gold Nanoclusters（AuNCs）is synthesized by using a gold stock solution etched with gold nanoparticles into small nanoclusters. In this study, AuNCs were bioconjugated with dihydrolipoic acid（DHLA）for protection the AuNCs assemble, the particle size is around 1 - 2 nm which make the AuNCs to have the red fluorescent. Previous study has demonstrated that preincubation of AuNCs with mouse blastocysts has no cytotoxicity. Here, we want to further investigate the effects of AuNCs on development of mouse two cell and morula stage of embryos. Two-cell stage embryos incubated with AuNCs（0 - 20 nM）for 48 hr, with different concentrations of embryos was found to have red fluorescence, it showed that AuNCs can localized into the embryos. In this result, it showed that the embryos incubated with 10 and 20 nM of AuNCs cannot develop to blastocyst after 48 hr. In the Hoechst staining assay, we found that the total cell numbers of embryos for incubation with various concentrations of AuNCs have the significant different with control group. it shows that AuNCs have the hazardous effects on the mouse early stages embryos. In the future, we will study the effects of AuNCs on intracellular ROS changes and various stages embryo development processes in early stage embryos and mouse embryonic stem cells.