Effects of PAMAM Dendrimer Generation 2 and 3 on ear1y mouse embryonic development

碩士 === 中原大學 === 生物科技研究所 === 102 === ABSTRACT PAMAM dendrimer has been recently shown can deliver drug or gene into the targeted mammalian tissues. However, certain studies revealed that PAMAM dendrimers impaired mammalian cells. Moreover, in terms of teratogenicity study little information focused...

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Main Authors: Sabuna-Alan Charis, 夏安良
Other Authors: Wen-Hsiung Chan
Format: Others
Language:en_US
Published: 2014
Online Access:http://ndltd.ncl.edu.tw/handle/64560301182024127108
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spelling ndltd-TW-102CYCU51110102015-10-13T23:49:48Z http://ndltd.ncl.edu.tw/handle/64560301182024127108 Effects of PAMAM Dendrimer Generation 2 and 3 on ear1y mouse embryonic development 第二代和第三代樹狀高分子PAMAM對小鼠胚胎發育的影響 Sabuna-Alan Charis 夏安良 碩士 中原大學 生物科技研究所 102 ABSTRACT PAMAM dendrimer has been recently shown can deliver drug or gene into the targeted mammalian tissues. However, certain studies revealed that PAMAM dendrimers impaired mammalian cells. Moreover, in terms of teratogenicity study little information focused on the cytotoxicological study of PAMAM dendrimers on mammalian embryos. Thus, this study is conducted to examine the influence of generation 2 (G2) and generation 3 (G3) of PAMAM dendrimer on mouse blastocyst. To investigate the influence of PAMAM-induced cytotoxicity, cell morphology, apoptosis, proliferation, in vitro development, reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP) change were examined. The results indicate that a generation dependent cytotoxicity of the PAMAM dendrimers was found to be correlated well with the number of amine group at surface area (G2<G3). Nevertheless, PAMAM dendrimer G2 showed only minor toxicity with low ability to induce apoptosis, low suppress on cell proliferation and small effect on in vitro development. Interestingly, the intracellular ROS level and mitochondrial membrane potential on mouse blastocyst was not altered by PAMAM dendrimer G2. This study provided an important insight that PAMAM dendrimer G2 has low cytotoxicity at the cellular level. Wen-Hsiung Chan 詹文雄 2014 學位論文 ; thesis 72 en_US
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language en_US
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description 碩士 === 中原大學 === 生物科技研究所 === 102 === ABSTRACT PAMAM dendrimer has been recently shown can deliver drug or gene into the targeted mammalian tissues. However, certain studies revealed that PAMAM dendrimers impaired mammalian cells. Moreover, in terms of teratogenicity study little information focused on the cytotoxicological study of PAMAM dendrimers on mammalian embryos. Thus, this study is conducted to examine the influence of generation 2 (G2) and generation 3 (G3) of PAMAM dendrimer on mouse blastocyst. To investigate the influence of PAMAM-induced cytotoxicity, cell morphology, apoptosis, proliferation, in vitro development, reactive oxygen species (ROS) generation, mitochondrial membrane potential (MMP) change were examined. The results indicate that a generation dependent cytotoxicity of the PAMAM dendrimers was found to be correlated well with the number of amine group at surface area (G2<G3). Nevertheless, PAMAM dendrimer G2 showed only minor toxicity with low ability to induce apoptosis, low suppress on cell proliferation and small effect on in vitro development. Interestingly, the intracellular ROS level and mitochondrial membrane potential on mouse blastocyst was not altered by PAMAM dendrimer G2. This study provided an important insight that PAMAM dendrimer G2 has low cytotoxicity at the cellular level.
author2 Wen-Hsiung Chan
author_facet Wen-Hsiung Chan
Sabuna-Alan Charis
夏安良
author Sabuna-Alan Charis
夏安良
spellingShingle Sabuna-Alan Charis
夏安良
Effects of PAMAM Dendrimer Generation 2 and 3 on ear1y mouse embryonic development
author_sort Sabuna-Alan Charis
title Effects of PAMAM Dendrimer Generation 2 and 3 on ear1y mouse embryonic development
title_short Effects of PAMAM Dendrimer Generation 2 and 3 on ear1y mouse embryonic development
title_full Effects of PAMAM Dendrimer Generation 2 and 3 on ear1y mouse embryonic development
title_fullStr Effects of PAMAM Dendrimer Generation 2 and 3 on ear1y mouse embryonic development
title_full_unstemmed Effects of PAMAM Dendrimer Generation 2 and 3 on ear1y mouse embryonic development
title_sort effects of pamam dendrimer generation 2 and 3 on ear1y mouse embryonic development
publishDate 2014
url http://ndltd.ncl.edu.tw/handle/64560301182024127108
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