The putative roles of huMETCAM in modulating the development and progression of nasopharyngeal carcinoma

碩士 === 中原大學 === 生物科技研究所 === 102 === METCAM, a cell adhesion molecular belong immunoglobulin-like gene superfamily, is located on the cell membrane glycoprotein. We have found in our past studies that METCAM has different functions in different cancers. METCAM expression promotes the progress of mela...

Full description

Bibliographic Details
Main Authors: Yen-Chun Liu, 劉彥君
Other Authors: Guang-Jer Wu
Format: Others
Language:zh-TW
Published: 2014
Online Access:http://ndltd.ncl.edu.tw/handle/31502027499038877850
Description
Summary:碩士 === 中原大學 === 生物科技研究所 === 102 === METCAM, a cell adhesion molecular belong immunoglobulin-like gene superfamily, is located on the cell membrane glycoprotein. We have found in our past studies that METCAM has different functions in different cancers. METCAM expression promotes the progress of melanoma, breast and cancer and prostate cancer. On the other hand, METCAM expression suppresses ovarian cancer proliferation and metastasis. We found that METCAM expression in NPC patients is very interesting. METCAM is expressed in normal nasopharyngeal tissue, but decreases expression in nasopharyngeal carcinoma and re-expressed in metastatic lesions. To understand METCAM’s function in NPC, we transfected METCAM cDNA gene into NPC-TW01 established from Type I NPC and NPC-TW04 established from Type II NPC cells and isolated G418R clones to be tested in various in vitro and in vivo experiments. Our results showed that METCAM over-expression had no effect on in vitro cell growth rate of both NPC-TW01 and NPC-TW04 cell lines. METCAM over-expression in NPC-TW01 deceased in vitro cell migration and invasion ability, and inhibited in vivo tumor growth in athymic nude mice. But opposite results were obtained in NPC-TW04: METCAM expression in NPC-TW04 increased in vitro cell migration and cell invasion ability, and increased in vivo tumor growth in athymic nude mice. We suggest that METCAM is a tumor suppressor gene in Type I NPC-TW01, but as a tumor promoter gene in Type II NPC-TW04. Preliminary mechanistic studies showed that METCAM expression decreased NPC-TW01 proliferation, angiogenesis and survival pathway, but increased apoptosis, in contrast, it increased NPC-TW04 proliferation, angiogenesis and survival pathway, but decreased apoptosis.