Preparation of the biocompatible silica nanofibers by an electrospinning method for application of nerve tissue engineerings

• Main Authors: Yu-Shan Chien, 簡郁珊
• Other Authors: Yui-Whei Chen
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/8t82bv

碩士 === 中原大學 === 化學研究所 === 102 === In this study, the silica nanofiber was prepared via the sol-gel polymerization by the electrospinning method. In the process, polyvinylpyrrolidone (PVP) was mixed with the silica precursor as a thickener to form the electrospinnable solution for the electrospinning process. The polymer was then removed by the calcination to obtain the porous silica nanofiber (SNF). Subsequently, the as-prepared SNF was modified with (3-aminopropyl) trimethoxysilane (APTS) to form the amino functionalized SNF (SNF–AP3) for application in the neural tissue engineering. SNF–AP3 was further modified by sulfosuccinimidyl–4–(N-¬maleimido-methyl)¬¬cyclohexane–1–carboxylate (Sulfo-SMCC) as a crosslinking agent, followed by grafting Laminin, a growth factor for neuron stem cell, on the SNF-AP3 fiber. The as-prepared chemically grafting Laminin SNF (SNF-AP3/SL) was then compared to the physical adsorpted SNF-AP3 fiber (SNF-AP3/L) for the impact on the growth and differentiation of neural stem cell. In addition, pure silica nanofibers (PSNFs) were also successfully prepared by the electrospinning method with the silica precursor solution without polymer thickener. All the structures and properties of the as-prepared SNF, the modified SNF (SNF–AP3、SNF-AP3/L and SNF-AP3/SL) and PSNFs were confirmed by a combination of FTIR, TGA, nitrogen adsorption/desorption isotherms, contact angle analysis and SEM measurements. The distributions of the laminin on the surface of SNF-AP3/L and SNF-AP3/SL fibers were investigated with the immunofluorescence technique. The results showed that the neural stem cells could effectively differentiate into neurons on both SNF-AP3/L and SNF-AP3/SL. Therefore, these two materials have the potential as biological scaffolds. Besides, the degradation behaviors of the as-prepared pure silica nanofibers (PSNF) were compared with that of the SNF series materials. The results indicated that the SNF series fibers were biodegradable, while PSNF series is relatively insoluble due to non-porous structure. In the future, a porous PSNF may be obtained by using non-toxic and water soluble materials as templates, leading to be potential of biodegradable materials.