Improvement and Performance Testing of Bioaerosol Generation & Sampling

• Main Authors: Wei-Ting Lin, 林威廷
• Other Authors: 賴全裕
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/65048471098355593316

碩士 === 中山醫學大學 === 職業安全衛生學系碩士班 === 102 === Andersen N6 single-stage bio-aerosol sampler is an impact sampler. In the process of collecting the sample, many microorganisms may die because of the impact. If the density of the sample is too high, there may be the masking effect. Therefore, the purpose of this study is to develop a bio-aerosol generator, to improve the efficiency of the Andersen single-stage sampler, and to develop the dilutor for the sampling and studies its efficiency. This study used ultrasonic orifice atomizer to generate its mist/fog for its bio-aerosol generator. It studied the outcomes of different power on four different sizes of orifice of atomizer plates. This study further modified the Andersen single-stage sampler by using a 15cm culture medium, and diluted the dilutor ten times. Results showed that atomizer plate with 7μm set at 10V, 0.22A could have a more stable and higher concentration of bio-aerosols than Collison three-jet nebulizer at 15psi. However, the most efficient nebulization on the four different atomizer plates was not with the maximum power but the second highest power. By using 15cm culture medium on the Andersen single-stage bio-aerosol sampler in the collecting of the sample, the efficiency could increase as much as 24.2% and in addition there were three different kinds of bacteria found,--Arcanobacteriumpyogenes, Corynebacteriumamycolatum, and Enterococcus hirae. In the sampling, Staphylococcus species could increase 42-69% in density but Micrococcus species decreased its density to about 1/4. On the sampling of fungi, the density of Penicilliumcitrinum increased 3 times and the density of Cladosporiumoxysporum increased 1.6 times. There was about 22% loss of bio-aerosols because of vessel wall.