| Summary: | 碩士 === 中山醫學大學 === 營養學系碩士班 === 102 === Hyperglycemia from diabetes promotes glucose to polyol pathway flux, which in turn causes tissue damage through oxidative and inflammatory stress. Solanum muricatum Ait (pepino) is a popular plant food in Penghu, Taiwan. Our previous study has reported that the aqueous extract from pepino was able to enhance antioxidant defense and decline polyol pathway in streptozotocin induced type 2 diabetic BALB/c mice. Therefore, we further used high-fat diet and streptozotocin induced type 2 diabetic BALB/c mice as a model to investigate the protective effects of pepino aqueous extract. Diabetic mice were fed by 1 or 2% aqueous pepino for six weeks. The serum and tissues were collected to analyze. Results showed that pepino intake improved hyperglycemia, insulin resistance and dyslipidemia. This extract decreased the activity of aldose reductase, a key enzyme in polyol pathway in kidney. Also, the accumulation of glycoxidation products, fructose and N-ε-carboxymethyl-lysine levels were reduced. In addition, this extract significantly reduced malondialdehyde level, retained glutathione content and glutathione peroxidase activity in liver. Pepino intake also increased total antioxidant capacity in liver, kidney and pancreas, attenuated oxidative damage. Proinflammatory cytokines such as TNF-α、IL-1β and IL-6 produced by adipose tissue were also decreased. In addition, pepino intake up-regulated PPARγ and increased gastric inhibitory polypeptide secretion. These results suggested that pepino aqueous extract could prevent progression of high-fat diet and streptozotocin induced type 2 diabetic complications by decreasing oxidative stress, glycosylation and inflammatory response.
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