Gellan gum hydrogel as the matrix of 3D tumor spheroids for cancer research platform

• Main Authors: Pin-Chun Liu, 劉品君
• Other Authors: Ming-Wei Lee
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/3v3x8q

碩士 === 中山醫學大學 === 醫學檢驗暨生物技術學系碩士班 === 102 === About oral cancer pathogenesis, more research is focused on the chemical factor induced cell proliferation and progression, however, physical stimulation may also induce reasons. Relations mechanical stimulation and cancer in breast cancer, oral cancer aspects have wanted more research. In the past, construction of 3D culture system of cancer cells in vitro using Matrigel as a substrate, this study is based on microbial polysaccharides Gellan gum hydrogel to construct 3D cell culture system. Gellan gum form gels with divalent cations, preparation of 0.6% and 0.8% of Gellan gum with 0.5% CaCl2 to become a 3D structure of the gel, and co-cultured with the oral cancer SCC15 (squamous cell carcinoma 15) to form tumor sphere. By WST-1 test preliminary experimental results are shown in 3D and 2D culture 24 hrs, 48 hrs and 72 hrs after, materials for oral cancer SCC15 has no significant differences. In addition, the 3D hydrol gel was also used as a fat-soluble drugs and water-soluble drugs of drug transport carrier. Confirmed by the findings, Gellan gum gum as water soluble drug transport vector, 3D structures will disintegrate in 48 hours; Encapsulating of a lipophilic drug, Gellan gum structure can be maintained over two weeks. The research results will be applied in the future of the in vitro research platform and oral cancer treatment.