| Summary: | 碩士 === 中山醫學大學 === 醫學檢驗暨生物技術學系碩士班 === 102 === Bladder cancer is a very frequent and aggressive malignant tumor. Bladder cancer is difficult to detect at early stages, and is usually at the late stages when diagnosed. In Taiwan, bladder cancer is the ninth most frequent malignancy diagnosed in men and the 16th in women based on a 2011 report from the Department of Health, Republic of China. Miconazole (MIC) is an imidazole antifungal agent, in recently reports, MIC have been shown to inhibit a variety of cancers, for example, breast cancer, mammary cancer and osteosarcoma. But there are not any research about bladder cancer. The aim of this study was to determine the effects of MIC on the growth inhibition and apoptosis of human bladder cancer cell. In the study showed that MIC elicited cytotoxic effects on human TSGH 8301 bladder as determined by MTT assay. The drug also impact on cell cycle confirmed by flow cytometry. MIC could induced DNA fragmentation formation and Annexin V-FITC / PI stained, toward to mitochondria-mediated cell apoptosis pathway as determined by JC-1 stain. Western blot analysis revealed that the increases of p21 and p27 protein levels, alone with the decrease of Cyclin E1, CDK2 and CDK4 expressed in MIC-treated TSGH-8301 cells, activation of caspase-3/-9, and cleavage PARP, and cytochrome c and SMAC protein increased from cytoplasm. These results indicate that the mitochondrial pathway is involved in MIC-induced growth inhibition and apoptosis of human bladder cancer, suggesting that MIC may be a potential anti-bladder cancer agent in human.
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