| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 102 === Chinese women in the top ten causes of death in cancer, cervical cancer is seventh, 10 years standardized mortality rates of cervical cancer in females with the largest decline, should be attributed to the Republican National Health Board since 1995, providing Pap smear screening after the inspection, so that the mortality rate declined by about Liu Cheng. However, cervical cancer remains the first female reproductive tract cancers. Berberine is a plant alkaloid with a long history of medicinal use in Chinese medicine. The berberine alkaloid can be found in the roots, rhizomes, and stem bark of the plants.Berberine extracts and decoctions have demonstrated significant antimicrobial activity and has several anti-inflammation and anti-cancer biological effects. However, its mechanism of action on the cell migration and invasion of human cervical cancer cells is not fully understood. Human cervical cancer cells were treated with berberine, subjected to invasion assay, motility assay and MTT assay, significantly inhibits invasive, motility in human cervical cancer cells. Zymography and promoter luciferase analysis revealed that berberine inhibits the proteinase and transcription activities of MMP-2 and u-PA, respectively.We also use NF-κB inhibitor to find that berberine inhibited NF-κB which then led to the inhibition MMP-2 expression. Overall, berberine on transcriptionally regulation of MMP-2 expression via the down-regulation of NF-κB followed by the inhibition of migration and invasion in human cervical cancer.
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