Effects of Black Tea Ethanol Extracts on Invasion and Proteinase Expression in SCC-4 Human Oral Cancer Cells

• Main Authors: Yi-Ping Huang, 黃怡萍
• Other Authors: Pei-Ni Chen
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/89752042421780949870

碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 102 === The incidence and mortality of oral cancer in Taiwan have been constantly increased during the last decade and this increase in mortality could be mainly resulted from the difficulty in treatment caused by metastasis. More than 90% oral cncers have been identified as squamous epithelium cell carcinoma, especially in tongue. And then, nearly 50% of patients with oral squamous cell carcinoma (OSCC) present with pathologic or clinical evidence of lymph node metastasis. Black tea, the most widely consumed beverage in the world, has been evidenced to possess prominent antioxidant ability. However, the detailed effects and mechanisms of black tea on cancer cell invasion and metastasis were still unclear. Therefore, SCC-4 cells, a malignant oral squamous carcinoma cell line, was used in this study. First of all, SCC-4 cells were subjected to a treatment with 50% ethanol extracts of black tea leaves. Furthermore, the chemical profile of black tea polyphenol extracts (BTE) was analyzed by using a high-pressure liquid chromatograph (HPLC)−mass spectrometer (MS). And then MTT assays. Results suggested that black tea extract(BTE) have no effect on cell viability of SCC-4 cells. The Transwell assay revealed that a treatment of BTE significantly inhibited the cell invasion (p < 0.001) and motility (p < 0.01) capacities of SCC-4 cells. Next, we used the cell-matrix adhesion assay to determine the cell adhesion capacity. Here, we found the BTE exerted an inhibitory effect on cell adhesion (p < 0.001) of SCC-4 cells. Therefore, the results of the cell spreading assay also showed that the same inhibitory effect on cell spreading of SCC-4 cells. Zymography and promoter luciferase analysis revealed that BTE inhibited the proteinase and transcriptional activities of MMP-2 and u-PA of SCC-4 cells, respectively. These results suggested that BTE may be beneficial prevention of cancer metastasis, and helpful development of cancer therapy.