| Summary: | 碩士 === 中山醫學大學 === 生化暨生物科技研究所 === 102 === Tumor metastasis is a complex multistep process and the major cause of cancer death and various treatment strategies have targeted on preventing the occurrence of metastasis. Epithelial to mesenchymal transition (EMT) is critical for the progression, invasion, and metastasis of epithelial tumorgenesis. Various treatment strategies have targeted the prevention of the occurrence of metastasis and reverse EMT. Studies have shown that tea polyphenol, have many anti-oxidant components and could inhibit tumorigenesis, including ovarian cancer and breast cancer. It has been showed that tea polyphenols may have potentially beneficial effects, including anti-metastatic, anti-inflammatory, anti-oxidant and apoptotic effects. However, effects molecular mechanism of black tea in human oral cancer is presently unknown. In this study, anti-metastasis, anti-tumor agents and reversion of EMT were investigated using black tea extracts (BTE), which was extracted by 50% ethanol, on a human malignant oral squamous cell line SCC-4. We demonstrated that BTE could inhibit the migration ability of SCC-4 by wound healing assay. Immunoblot was performed to find that BET could inhibit the proteins associated with cell adhesion ability such as p-paxillin、p-FAK and p-Src. BTE was down-regulation of calpain-2, a proteins associated with cell migrasion. Otherwise, mesenchymal marker vimentin expression was decreased. BTE could induce up-regulation of epithelial markers such as E-cadherin. Expression of transcription factor Snail-1 was decrease. Recomfirmed by immunofluorescence, Vimentin expression was significantly decreased. Found in gelatine zymography and casein zymography that BTE inhibits PMA-induced MMP-9 and u-Paexpression in SCC-4. BTE also inhibit the tumor growth of SCC-4 cells via cancer cell xenografted nude mice. We evidence, that BTE could reverse EMT and inhibit cancer growth ability in human oral cancer cells.
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