Genomic Mining of Aspergillus terreus: The Semi-Biosynthesis of Acetylaszonalenin and Its Analogues in anaPS Deletion Mutant

• Main Authors: Yi-Ling Chang, 張伊伶
• Other Authors: Kuan-Han Lee
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/h85vmt

碩士 === 嘉南藥理大學 === 藥學系 === 102 === A putative acetylaszonlenin-biosynthetic gene cluster was identified in the genome sequence of Neosartorya fischeri and Aspergillus terreus. This cluster consists of three genes, anaPS, anaPT, and anaAT. In the previous study, a anaPS strain of A. terreus NIH2624 was created and examination of the secondary metabolite profiles of the anaPS strain in comparison with the wild type strain clearly showed that acetylaszonlenin is missing in the deletion strain. N. fischeri NRRL181 and A. terreus NIH2624, and A. terreus anaPS strains are from Dr. Clay Wang’s laboratory in University of Southern California. The precursors of acetylaszonalenin, benzodiazepinedione, catalyzed by anaPS in the biosynthetic pathway of acetylaszonalenin were chemically synthesized. A. terreus anaPS strain was cultured and fed with benzodiazepinedione and its analogues of benzodiazepinedione. Secondary metabolites from N. flscheri and A. terreus were identified by HPLC and LC/MS. Acetylazonalenin was successfully obtained in A. terreus anaPS strain after feeding benzodiazepinedione. In this research, we successfully demonstrated the semi-biosynthetic approaches in the preparation acetylaszonalenin and its analogues. We keep on try our effort to optimize the growth condition for the bio-plant. The fungal genomic mining and biosynthesis of secondary metabolites will investigate continuously.