| Summary: | 碩士 === 嘉南藥理大學 === 保健營養系 === 102 === Epstein-Barr virus (EBV) is a human herpesvirus; and is associated with several human lymphoid malignancies. Our earlier study found that the ethanolic extract of Polygonum cuspidatum root promotes apoptosis of EBV-positive cells due to its ability to inhibit the expression of latent membrane protein 1 and deplete NF-B in the nucleus. Therefore, the purpose of this study is to investigate the effects of the ethyl acetate fraction F3a from P. cuspidatum on Raji cells. Trypan blue assay was used to determine the viability of Raji cells; real-time quantitative PCR to assess the expression of EBV mRNA and EBV DNA in Raji cells; to measure ROS production using by DCFH-DA kit. The expression of LMP1, apoptotic-related proteins, ERK and IB were determined by western blotting. Flow cytometry analysis was used for the detection of apoptotic cells. Results show that F3a promotes Raji cells death, with a CC50 were of 12.08 g/ml F3a at 12.5 g/ml induces transcription of BRLF1 and BNLF1 and increases the expression of LMP1, which reduces intracellular phospho-ERK and phospho- IB expression. Meanwhile, F3a increases reactive-oxygen species, actived apoptotic-related proteins, caspase 3, cleavage of PARP, and increases the percentage of apoptotic cells. Therefore, F3a can be developed as a therapeutic drug in the treatment of EBV-related tumors.
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