Anti-diabetic activities of chrysophanic acid and it's molecular mechanism (s) of action in mouse C2C12 cells

• Main Authors: Yu-Ching Sun, 孫于晴
• Other Authors: Shu-Jing Wu
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/z735xp

碩士 === 嘉南藥理大學 === 保健營養系 === 102 === Abstact In 2012, diabetes mellitus (DM) was ranked fifth among the top 10 causes of death in Taiwan, and more than 95% at the DM patient found in Taiwan are type 2 DM. Chrysophanic acid is one of the organic acids, that possesses antibacterial, anti-cancer and other physiological activities. In this study, cell viability, the glucose absorption, the production of ROS, as insulin resistance, Akt/PI3K, as well as AMPK sigualing transduction pathways ware examine in the C2C12 cells. The results revealed that chrysophanic acid displayed eternity against cell toxicity, up-regulated the absorption of glucose as well as down-regulated the ROS formation in a dose-dependent manner. In addition, chrysophanic acid significantly increased the expression of IRS-1, p-IRS-1, PI3K, p-Akt, and p-AMPK genes. These results indicate that chrysophanic acid may play an important role in the anti-diabetic activity through inhibiting the production of ROS, as well as activation of IRS-1 and PI3K proteins, and phosphorylation of Akt and AMPK proteins.