Extract of Auricularia polytricha improved bone metabolisim by inhibition of mevalonate pathway

• Main Authors: Tzu-Yi Chen, 陳慈佾
• Other Authors: Hui-Ting Yang
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/t28vae

碩士 === 中國醫藥大學 === 營養學系碩士班 === 102 === Of all edible mushrooms, Auricularia Polytricha has a similar shape with Auricularia auricularia, and became the most common edible fungi in Taiwan. Auricularia genus contains a lot of calcium, polyphenols and non-soluble fiber. The previous experiment in our laboratory proved the effects of long-term intake APE (A. polytricha extract) improved the menopausal bone loss of ovariectomized female rats. Therefore, the probable mechanism in this study was further investigated. The 48 six-week-old female SD rats were randomly divided into six groups. There were the Sham group, the OVX (ovariectomy) group, the OVX+L (APE freeze-dried powder 0.05 g/kgw) group, the OVX+M (APE 0.1 g/kgw) group, the OVX+H (APE 0.3 g/kgw) group, and the OVX+G (APE spray-dried powder 0.3 g / kgw) group. During the breeding, we monitored the changes in body weight and food intake. At week 24 we collected the blood samples for analysis of the bone metabolism markers and took femur slices for analysis of bone mass density. The results of the liver and renal function showed that there were no differences of serum AST and ALT between groups. Using APE groups also didn’t show significant difference in BUN and Cr with the Sham group. With increasing age, we found out that groups with APE supplementation had a downward trend in bone-specific alkaline phosphates, a decrease of osteocalcin levels in the blood, but the osteoprotegerin had a significant increase in a dose-dependent manner. The trabecular bone analysis and three-dimensional imaging analysis also showed that OVX+G group increased significantly. According to the results, we found out that different processes of the APE will improve their effectiveness. Long-term intake of APE spray-dried powder 0.3 g/kgw for the postmenopausal female rats showed to promote bone turnover, increased osteoprotegerin activity, reduced isoprenylated proteins production by inhibiting the farnesyl pyrophosphate synthase activity, and reduced osteoclast activation and differentiation to enhance bone density.