The study of molecular mechanism underlying the anti-tumor effect of Malabaricone A in oral cancer cells

• Main Authors: Tzu-Fan Hsin, 辛子帆
• Other Authors: Ju-Hwa Lin
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/bgkmha

碩士 === 中國醫藥大學 === 生物科技學系碩士班 === 102 === The mortality of oral squamous cell carcinoma (OSCC) is one of the ten leading causes of cancer deaths in Taiwan. Environmental carcinogens such as betel quid chewing, tobacco smoking and alcohol drinking have been identified as major risk factors for OSCC. The fruit rind of the plant Myristica malabarica (Myristicaceae), popularly known as rampatri, Bombay mace, or false nutmeg is used as an exotic spice in various Indian cuisines. Its pharmacological activities range from hepatoprotective, anti-ulcerogenic to anti-cancer. The methanol extract of M. malabarica has also been shown to have superoxide scavenging, prolyl endopeptidase inhibitory activity and antioxidant activity. There are four members in Malabaricone family Malabaricone A-D (Mal A-D), obtained from the methanol extract of Myristica malabarica. In this study, we investigated the anti-cancer activity of Malabaricone A (Mal-A) and its molecular mechanisms in the oral squamous cell carcinoma cell line Ca922. Mal-A inhibited Ca922 cell viability in a dose- and time-dependent manner by MTT assay. Treatment with 25、50 and 75 μM of Mal-A for 48 h led to DNA damage and apoptosis by DAPI staining. We also show that Mal-A reduced cell viability and induced cell death though the induction of apoptosis which was determined by PI and Annexin V assays. Taken together, these findings provide important new possible molecular mechanisms for the anti-oral cancer activities of mal-a on OSCC cells.