Developing a Porcine Burn Model for Studying the Effects of Jin-Chuang-Gao on Second Degree Burns

• Main Authors: Shi-Yuan Sheu, 許世源
• Other Authors: 姚俊旭
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/sw7p97

博士 === 中國醫藥大學 === 中醫學系博士班 === 102 === Objective： In this study, we investigate the beneficial effects on experimental wound healing with a new porcine burn model. The second-degree burn wound model could meet the clinical uses of wound dressing for conservative treatment needs. Background： Tian-Xia-Di-Yi-Jin-Chuang-Gao, a famous ancient formula composed of rosin, mastic, myrrh, catechu, dragon''s blood, camphor, borneol, starch, beeswax and lard, was first described in Yi Xue Xin Wu by Guo-peng Cheng in China in AD 1732. Traditionally, the ointment has been widely used in clinical medication for the treatment of traumatic wounds, pressure ulcers, diabetic wounds, and corrosive injuries. In the previous study, Chang et al. (2010) have provided supporting evidence to facilitate wound healing at 21 postburn days in a porcine burn model. Materials and Methods： Castrated male swine (Landrace, 2 months old) weighing 20 kg were used in this study. Animals were subjected to burns while under anesthesia and analgesia with a combination of atropine (0.06 mg/kg), Zoletil® 50 (4.4 mg/kg) and xylazine (2.2 mg/kg) by intramuscular injection. This model used stainless steel round bar of a diameter of 30 mm and a height of 30 mm, weighting 230.9±b0.4 g. The bar was heated up to 80-110ºC with a high-performance hotplate. By varying the heated temperature applied on the bar, a 1 kgf mechanical force was applied for a period of 20 s using a push pull gauge. All burns were covered with moist saline dressings and daily wound treatments were performed under general anesthesia by sterile normal saline solution washing, which removes the coagulate tissue and the residual viable structures; animals were treated by xylocaine jelly 2%, P1, P2, P3 and P4 dressing, and covered with 3” x 3” gauze sponge and followed for a period of 24 days. A 6 mm punch biopsy was taken at different day post-burn for histological evaluation using HE stain and Masson trichrome. The correlation between clinical assessment and photographic evaluation was also calculated. Results： As noted in this study, the difference in temperature (ΔT) that before and after creating the burns are of relevance in the areas of burn wounds evidently. After creating the burns, the histological studies have shown a non-linearly correlation between the collagen changes in wounds and the depth of burns. The quantity of the collagen has a major role affecting the rate and quality of wound healing.