Using Ionic Wind Driven Micro-centrifugal Device to Trap Bacteria Recognized by Functionalized Raman Reporter Labeled Beads

• Main Authors: LIN, SHIH YUN, 林詩芸
• Other Authors: 王少君
• Format: Others
• Language: zh-TW
• Published: 2014
• Online Access: http://ndltd.ncl.edu.tw/handle/33918018653725903730

碩士 === 國立中正大學 === 化學暨生物化學研究所 === 102 === Micro-centrifugal force driven by ionic wind generated near a corona needle tip has been an emerging micro-fluidic technique to concentrate and trap suspended particles of micron size. The surrounding air near the corona needle is ionized when high ac voltage is applied. The accumulated gas ions are repelled from each other at the needle tip to eject and produce ionic wind, which can swipe across the liquid air interface inside a miniaturized reservoir to generate vortices. Suspended particles are further dragged into the reservoir center, where the vortex is more intense to trap at the reservoir bottom. In this thesis, polystyrene micro-beads and fluorescent microspheres were used to find out the suitable trapping conditions, including to try the angle (φ) between needle and surface and solution ionic strength. In addition,, the effects of particle size in centrifugal aggregations was also studied. Functionalized nanoaggregate-embedded beads (NAEB), made of silica-coated gold nano-particle aggregates doped with Raman dyes. Antibodies immobilized on NAEB surface were able to recognized antigen-functionalized PS beads, the tokens of baterials. The NAEB-PS bead conjugates were trapped with the ionic wind device and using Raman microscopy to observe. Finally the ionic wind device was also use to trap Salmonella baterials docked with antibody-functionalized NAEB to demonstrate the potential applications in food analysis.