Microwave inhibits gene expression of inflammatory factor NFκB in THP-1 derived macrophages

• Main Authors: Shen Sheng Wei, 沈昇偉
• Other Authors: Ting-Yu Kao
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/yw2nyk

碩士 === 元培科技大學 === 醫學檢驗生物技術研究所 === 101 === Microwave is a non-ionizing radiation. We have inevitably exposed to microwave radiation because of utilization of microwave ovens and radio communication equipments. Recently, World Health Organization (WHO) has warned that microwave interferes with immune function and induces carcinogenesis, such as leukemia and brain tumors. However, few literatures reported how microwave irradiation affects the immune function. The aim of this thesis is to explore how microwave affects immune system through inhibition of gene expression of inflammatory factor NFκB in macrophage and how curcumin, as a immune modulator, affects gene expression of NFκB. In our study, we used PMA to stimulate differentiation of human monocytic cell line THP-1 into macrophage, followed by activation of NFκB p65 and immune response through LPS stimulation. We estimated gene and protein expression of NFκB p65 in macrophage by using real-time quantitative PCR and Western blot analysis. When macrophage was treated by LPS, IκBα was degraded by proteasome system and its partner NFκB p65 would translocate into the nucleus and drive gene expression of cytokine interleukin-1β (IL-1β). However, under microwave irradiation (2450 MHz, 900w), we found that microwave decreases the mRNA and protein level of NFκB p65 in a dose-dependent manner. Indeed, microwave also inhibited the secretion of cytokine interleukin-1β (IL-1β). On the other hand, curcumin restored the inhibitory effect of microwave on mRNA and protein expression of NFκB p65 in macrophage. Under microwave irradiation, curcumin increased NFκB p65 gene expression at 20 to 30 to 40 μM an. In summary, our results show that microwave inhibits gene expression of NFκB p65 in macrophage and subsequent immune response; while curcumin restores suppressed gene expression of NFκB p65 and immune response in macrophages due to microwave irradiation.