Differentiation of induced pluripotent stem cells in different materials into myocardial cells under hypoxia condition

• Main Authors: Chiu-hui Sung, 宋秋慧
• Other Authors: Taz-wen Chung
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/11596314291051575142

碩士 === 國立雲林科技大學 === 化學工程與材料工程系碩士班 === 101 === This study is to promote induced pluripotent stem cells to differentiate into cardiomyocytes by using hypoxia condition with biodegradable materials polycaprolactone (PCL) and having the biocompatible material such as gelatin, heparin, chitosan and hyaluronic acid and BMP4 growth factors and materials Ascorbic acid. The experimental results show that the surface has been completely PCL material chemically modified, with Gelatin, Heparin, HA and CS in different material combinations on the PCL material. According to the contact angle of hydrophilic materials such as: the contact angle of HA and Heparin is lower than PCL, and also the ATR-FTIR functional group is used to know the materials having been successfully cross-linked on the PCL material. In vitro cell experiments, the relationships between the materials and cell adhesion and proliferation have not been defined yet. In differentiation part, according to the preliminary results by immunofluorescent staining, the group of Heparin with HA and PCL material does enable myocardial protein expression which was higher than gelatin; it is found that hypoxia condition can promote induced pluripotent stem cells into cardiomyocytes compared to the group cultured under normal condition.