Investigation of the Functional Role of Metadherin (MTDH) in Lung Adenocarcinoma

• Main Authors: Jian-Chiao Wang, 王建喬
• Other Authors: Wailap Victor Ng
• Format: Others
• Language: zh-TW
• Published: 2013
• Online Access: http://ndltd.ncl.edu.tw/handle/05182589439018450904

碩士 === 國立陽明大學 === 醫學生物技術暨檢驗學系 === 101 === Abstract According to the World Health Organization (WHO) statistics, cancer is a leading cause of death worldwide. In 2008, International Agency for Research on Cancer (IARC) published the number of incidence of lung cancer ranks the third, and mortality of lung cancer is the first. In Taiwan, the mortality of lung cancer is the first, too. Lung cancer can be classified in two category, non-small-cell lung carcinoma and small-cell lung carcinoma. Non-small-cell lung carcinoma can be classified in three subtypes, i.e., adenocarcinoma, squamous-cell lung carcinoma and large-cell lung carcinoma. Among these, the most common type is adenocarcinoma. Although there are a number of options available for the treatment of lung cancers, e.g., surgery, chemotherapy, radiotherapy, and target therapy, when lung cancers start to metastasize, they usually have poor prognosis. There are many reports which showed cancer metastasis is related to abnormal expression of cell membrane proteins in the past few years. Previously, a former labmate Pei Yin Shih performed a proteomic analysis of the cell membrane proteins of two related lung adenocarcinoma cell lines. She found 28 proteins might have higher levels in the relatively higher metastatic CL1-5 than the lower metastatic CL1-0. Among these, previous studies suggested eleven proteins including MTDH, PROCR, and HMOX1… etc., may be related to metastasis and certain type of cancers. However, the roles of MTDH in lung adenocarcinoma remain unclear. In this study, we explored its role by using lentivirus system and shRNAs to knockdown MTDH expression in CL1-5, and transfected MTDH plasmid into CL1-0 to overexpress the protein. Interestingly, when the MTDH in CL1-5 was knockdown, the cells became more like the CL1-0 as suggested by the cell morphology and wound healing analysis. When MTDH is overexpressed in CL1-0, the cells became more like CL1-5.Now, the MTDH knockdown and overexpressed cells are available for proteomic analysis to study its roles in lung adenocarcinoma.