| Summary: | 博士 === 國立陽明大學 === 微生物及免疫學研究所 === 101 === Background and objectives: Human epidermal growth factor receptor-2 (HER2) is overexpressed in 25%-30% of breast cancers. HER2 is preferentially localized in the lipid rafts, in which a major component is stomatin. This study aimed to elucidate the role of stomatin expression to the progression of HER2-positive breast cancer.
Methods: The BT474 and SK-BR-3 are HER2-overexpressing breast cancer cell lines. The cell proliferation, migration and invasion were tested under overexpression and down-regulation of stomatin. Imunohistochemical staining of tissue samples from patients of infiltrating ductal carcinoma of breast were analyzed.
Results: When stomatin is overexpressed in BT474, the proliferation and migration ability of the cells decreased. On the contrary, when stomatin was knocked down in the SK-BR-3, cell proliferation and migration were increased. In a total of 35 clinical cases of HER2-positive breast cancer, the absence of stomatin expression correlated with an older age, a more advanced stage, being estrogen receptor negative, and being progesterone receptor negative. The absence of stomatin expression was associated with a decreased 5-year-survival (14% vs. 93%, p&;lt;0.001) by survival analysis and was an independent prognostic factor by multivariate analysis.
Conclusion: These results suggest that stomatin is a prognostic indicator for HER2-positive breast cancer.
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