| Summary: | 碩士 === 國立陽明大學 === 生理學研究所 === 101 === Abstrat
Smoking caused physical stress and had been widely known for leading to chronic disease, such as cancer. According to studies, the relative risk for active smokers to develop adenocarcinoma is greater when comparing with non-smoking people. Acrolein (2-propenal), anα,ß-unsaturated aldehyde, exists in a wide range of sources. It is also present in a haze of cigarette smoke as well as the environment ubiquitously. Previous studies indicated that the immune system was correlated with physical stress, but the cell response by circulation in acrolein-pretreated rats was still unknown. It has been well known that the change of cytokine level appears to be an important initiated pre-immunity effect of the splenic monocyte (i.e. splenocytes) in rats. Although the toxicity of acrolein has been extensively studied, there is little information about its impact on stress induced immunity changes through cytokine release. This study aimed to explore the stimulatory effects of acrolein on the production of the tumor necrosis factor-alpha (TNF-α) in rat splenocytes. Splenocytes
were isolated and incubated with or without Lipopolysaccharide (LPS, 0~ 40 μg/ml) from rats, which was used as a positive control. In the present study, rat splenocytes were administrated in vitro with acrolein for
different doses (1x10-10 M~1x10-7 M). The results showed that the splenocytes exposure on acrolein resulted in a decrease of TNF-α release in vitro. Furthermore, the rats were administrated with acrolein (2 mg/ml/kg) for 1- or 3-day in vivo study. The results suggested that administration of acrolein decreased the release of TNF-α via an inhibition on monocytes in response to acute physical stress. The adrenocorticosteroid hormones with further speculation might be involved in changes of cytokine production in rats. In conclusion, these results suggested that the release of cytokines (e.g.TNF-α) from
splenocytes can be inhibited by the administration of acrolein in rats.
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